Effects of morphology on the characteristics of UC-MSCs. A Morphology of UC-MSCs of different sizes when they were attached to the culture dishes. Scale bar = 50 μm. B Morphology of UC-MSCs of different sizes when they were suspended in PBS. Scale bar = 100 μm. C Comparison of the survival rates of mice (n = 4 for each group) injected with UC-MSCs of different sizes. D Comparison of the proliferation potential of the two different sized UC-MSC populations. E Functional comparison of HLCs derived from UC-MSCs of the larger and smallerpopulations. Positive staining was quantified using ImageJ software, and the results were compared between the two populations. F Comparsion of the gene expression levels of cytokines (IDO, HGF, VEGF, TGF-β, IL-6, and IL-10) involved in immunity between larger and smaller UC-MSCs after stimulation with IFN-γ. All data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01; ns, not significant.