Fig. 2

Primary adult mouse sp-NSPCs cultured under different conditions show divergent differentiation. A Neurosphere formation in suspension cultures (bar = 50 μm) and sp-NSPCs in adherent cultures (bar = 100 μm). Cells were labeled with nestin-specific antibodies. B Immunofluorescent staining shows sp-NSPC differentiation into Tuj-1-positive neurons, GFAP-positive astrocytes, and O4-positive oligodendrocytes. Nuclei were stained with DAPI (blue) (bar = 100 μm). The analysis was calculated based on three biological replicates