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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: LINGO-1 regulates Wnt5a signaling during neural stem and progenitor cell differentiation by modulating miR-15b-3p levels

Fig. 4

Effects of LINGO-1 shRNA on sp-NSPC differentiation. A sp-NSPCs were transfected with GFP-expressing LINGO-1shRNA lentiviral vectors. B LINGO-1 mRNA and C LINGO-1 protein expression levels decreased significantly. Immunofluorescence staining and quantification demonstrated that D, E Tuj1+/GFP+ neurons increased significantly, while F, G GFAP+/GFP+ astrocytes decreased in LINGO-1 shRNA-treated sp-NSPCs. Enlarged areas show that astrocytes in the LINGO-1shRNA group appeared more mature (arrowheads) than those in the control group (arrows) (bar = 100 μm), *P < 0.05. Results are expressed as the mean ± SD. All of the analysis was calculated based on three biological replicates

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