Fig. 1

HSPCs were effectively isolated and purified by MACS. a Schematic illustrates the isolation of mouse bone marrow mononuclear cells (MNCs). b For lineage depletion, cells were magnetically labeled with a cocktail of biotinylated antibodies that are against a panel of so-called lineage antigens (CD5, CD45R (B220), CD11b, anti-Gr-1, and Ter-119 antibodies) and anti-Biotin MicroBeads. This labeling procedure leaves the lineage-negative cells undisturbed, thus allowing further separation of lineage⁻ cells. Then, the lineage⁻ cells (lin⁻ cells) were purified by c-Kit MACS, namely lin^-c-Kit⁺ cells. c HSPCs were cultured with a specific modeling medium (stem cell culture medium + 10 ng/mL IL3 + 10 ng/mL IL6 + 30 ng/mL SCF) and incubated for 8 days, its the model group. d Flow cytometric analysis of the purity of lin–c-Kit+ cells at different phases. The results were expressed as mean ± S.D., and the p values (*P < 0.05, **P < 0.01, ***P < 0.001) were determined by the ANOVA test