Fig. 2

Model HSPCs showed significant manifestations of aging such as increased β-galactosidase activity, G0/G1 phase arrest, decreased colony-forming capacity, and changes in cell morphology. a Photomicrographs of SA-β-gal staining(× 200). The percentage of SA-β-gal stain-positive cells was significantly increased in the model group compared with the young group, n = 10. b Flow cytometric analysis of cell cycle distribution. The model HSPCs were arrested in G0/G1 phase compared with the young group, n = 10. c Photomicrographs of CFU-Mix. The size and number of CFU-Mix significantly decreased in the model group compared with the young group. d. Photomicrographs of TEM. The nuclear membranes of young HSPCs were smooth and flat, chromatin evenly distributed; there were few to no inclusion bodies found in the cytoplasm. But the perinuclear cisternae in the model HSPCs widened, and chromatin edge aggregated. A large number of inclusion bodies appeared in the model HSPCs