Fig. 3

Integrated proteomic and transcriptomic analysis showed significant changes of metabolic processes associated with aging in Model HSPCs. a The volcano plot of proteomic, which plots significance versus fold change on the y- and x-axes, respectively, found upregulated and downregulated proteins using t-test. The red color represents the upregulated proteins, and the blue color represents downregulated proteins. b The heat map of proteomics showed protein expression levels of the young group and the model group. Color intensity indicates the level of expression, where green signifies low expression and red signifies high expression. c Gene Ontology analysis of proteomics, p < 0.05. d KEGG analysis of proteomics. Go enrichment (c) and KEGG pathway (d) analysis showed that glycolysis, lysosomal metabolism, ribosomal synthesis, and mRNA splicing were significantly changed in the model. e STRING analysis of proteomics showed that the common target proteins of PcG and TrxG are UHRF1 and TOPOIIa. f The scatter plot of transcriptomic: red dots were upregulated, and blue dots were downregulated. g Go enrichment analysis of transcriptomics showed genes associated with the stress response, inflammation, lysosomal metabolism, and protein aggregation dominated the upregulated expression profile, while the downregulated profile was marked by genes involved in the preservation of genomic integrity and chromatin remodeling