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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: CAR-engineered NK cells; a promising therapeutic option for treatment of hematological malignancies

Fig. 3

The common CAR constructions utilized in CAR-NK cell generation. CAR molecules on NK cells include three chief fragments, comprising an antigen detection domain (ScFv or NKG2D), and transmembrane domain concomitant with the signaling domain. First-generation CARs only include CD3ζ or DAP12 as the signaling domain, and CD3ζ seems to be a more efficient signaling domain than DAP10, while DAP12 can stimulate NK cell functions more powerful than CD3ζ. Besides, second-generation CARs express a second signaling domain, including CD28 or 4-1BB in association with CD3ζ. Finally, third-generation CARs include two costimulatory signaling domains. Importantly, respecting the mechanism by which NKG2D induces NK cells, an exclusive CAR construct including NKG2D as the ectodomain that connects DAP10 and CD3ζ as chief signaling domains has been progressed. Chimeric antigen receptor-natural killer (CAR-NK) cells, natural killer group 2D (NKG2D)

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