Fig. 3

The effects of MSC injection on insulin sensitivity and blood insulin levels. A Blood glucose levels from the last time of ITT after three injections of MSCs. After fasting for 5 h, blood was collected from the tail vein (time point 0), then the mice were intraperitoneally injected with insulin solution (1.5 U/kg). Blood was collected at 15, 30, 60, 90, and 120 min for blood glucose determination. Non-diabetic (ND) wild-type mice were included as healthy controls. “L (low)”, “M (medium)”, and “H (high)” represent the doses of injected cells. MET, metformin. B ITT was performed on the indicated groups to assess insulin sensitivity, and the results were presented as AUC of all 4 times of ITT among the groups. C Fasting plasma insulin levels before and after the MSC injection (day 42) were determined by Elisa test. The results are expressed as the means ± S.E.M. n = 6 mice per group. ^*P<0.05, ^**P<0.01, ^***P<0.001 vs vehicle. The colors of asterisks are consistent with the respective treatment groups and indicate the statistical significance versus the vehicle group. Significances between different doses of MSCs of the same tissue origin are indicated as ^#P<0.05, ^##P<0.01.Two-way ANOVA