Fig. 5

AKT/PI3K pathway agonist promotes proliferation and differentiation. A Representative images of H&E staining of lung tissue sections from Sox9^CreER; Rosa^tdTomato mice after RILI. An AKT agonist was intragastrically administered after radiation damage. Lung tissues were collected 3, 7 and 14 days after drug treatment. n = 5. Scale bar, 100 μm. B Immunostaining of a-SMA in mice receiving SC79 group and control group. n = 5. Scale bar, 100 μm. C, D Representative IHC staining images of ki67 and Caspase-3 after radiation damage and agonist treatment (left). Percentage of ki67+ and Caspase-3+ cells were assessed (right), respectively. **p < 0.01 and ***p < 0.001 as determined by unpaired Student’s t test. n = 5. Scale bar, 50 μm. E, F Representative images of SOX9⁺ cell-driven lineage tracing in mice after radiation injury and agonist treatment (left). Percentage of tdTomato⁺ cells was assessed (right), respectively. *p < 0.05, **p < 0.01 and ***p < 0.001 as determined by unpaired Student’s t test. Nuclei are labelled with DAPI (blue). n = 5. Scale bar, 50 μm