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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Spatio-temporal model of Meox1 expression control involvement of Sca-1-positive stem cells in neointima formation through the synergistic effect of Rho/CDC42 and SDF-1α/CXCR4

Fig. 3

Meox1 promoted Sca-1+ stem cell migration into neointima through activating Rho/CDC42 signaling. A Immunofluorescence (IF) double staining for Sca-1 and RhoA in injury arteries treated with or without Ad-shMeox1 for 14 days. Red fluorescence indicates Sca-1, green fluorescence indicates RhoA, and blue fluorescence indicates DAPI-labeled nucleus. I, neointima; M, media; A, adventitia. B RhoA expressions in neointima were determined within figure 3A by Image-Pro Plus software. n = 6, *P < 0.05 vs. sham; &P < 0.05 vs. the injured arteries treated with Ad-Null. C–D Rac1 expression in neointima were detected by IF staining (C) and analyzed by Image-Pro Plus software. Six rats each group, random three high power visual field each section, n = 6, *P < 0.05 vs. sham; &P < 0.05 vs. the injured arteries treated with Ad-Null. E CDC42 expression by IF staining; F CDC42 expressions in neointima were determined within E by Image-Pro Plus software. n = 18, *P < 0.05 vs. sham; &P < 0.05 vs. the injured arteries treated with Ad-Null. G, H Knockdown of Meox1 by shRNA reduced proteins levels of RhoA, Rac1, and CDC42 in the injured arteries transfected with treatment of Ad-shMeox1 for 14 days as determined by western blot (G) and semi-quantitative analysis (H). n = 3, *P < 0.05 vs. sham; #P < 0.05 vs. Ad-Null. I, neointima; M, media; A, adventitia

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Stem Cell Research & Therapy

ISSN: 1757-6512