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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Spatio-temporal model of Meox1 expression control involvement of Sca-1-positive stem cells in neointima formation through the synergistic effect of Rho/CDC42 and SDF-1α/CXCR4

Fig. 6

Meox1 triggered Sca-1-positive stem cell migration through RhoA-CDC42-CXCR4 signaling. The experiment was designed to demonstrate the role of RhoA-CDC42-CXCR4 axis in Meox1-mediated adventitia Sca-1-positive stem cell migration during neointimal formation, by observing human umbilical cord Sca-1-positive stem cells (HUCSCs) migration under transwell in vitro. A Immunofluorescence staining for CXCR4 and CXCR7. B Human umbilical cord Sca-1-positive stem cells (HUCSCs) migration with overexpression of Meox1. C Semi-quantitative analysis of HUCSCs migration with treatment of Ad-Meox1 or Ad-shMeox1. Three independent experiments were performed, n = 18, *P < 0.05 vs. HUCSCs treated with ad-Null; #P < 0.05 vs. HUCSCs treated with ad-Meox1. D, E Immunofluorescence staining for F-actin. Ad-Meox1 increased F-actin levels in HUCSCs, the specific effect could be abolished by CXCR4 blocker AMD3100, RhoA inhibitor CCG1423, CDC42 blocker ZCL278, or Rac1 inhibitor Azathioprine, especially in AMD3100 or CCG1423. F Meox1-mediated HUCSCs migration with the treatment of RhoA inhibitor CCG1423, CDC42 blocker ZCL278, or Rac1 inhibitor Azathioprine, respectively. Ad-Meox1 increased HUCSCs migration; the specific effect could be abolished by RhoA inhibitor CCG1423, CDC42 blocker ZCL278, or Rac1 inhibitor Azathioprine, especially in Azathioprine

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