Fig. 1

Metabolic studies in control and DIO mice. a, b Fasting blood glucose levels and body weight were determined every 4 weeks within 16 weeks. c–g The levels of serum TC, TG, FFA, AST, and ALT in control and DIO mice were detected by ELISA. h Liver steatosis were analyzed through staining with H&E (scale bar, 100 μm) and Oil Red O (scale bar, 200 μm). i H&E staining of skin (scale bar, 1 mm) and visceral fat (scale bar, 100 μm). j The quantification of lipid accumulation in h. k The quantification of thickness of subcutaneous fat layer in i. l The quantification of visceral adipocytes size in i. The data are expressed as mean values ± SD. n = 6 mice per group. *P < 0.05, **P < 0.01, ***P < 0.001