Skip to main content
Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Efficient hepatic differentiation and regeneration potential under xeno-free conditions using mass-producible amnion-derived mesenchymal stem cells

Fig. 8

Schematic illustration of the overall flow of this study. AM-MSCs were isolated and characterized. However, they had low levels of GATA6 and high levels of GSK3, which are reversed conditions for inducing definitive endoderm (DE). Accordingly, the efficiency of hepatic differentiation was low when the conventional protocol was used. To increase efficiency, CHIR99021 and several other factors were added to the definitive and hepatic endoderm induction medium. Also, FBS was substituted by PVA to provide xeno-free conditions for transplantation and enhance the efficiency of differentiation. After that, the maturation of hepatocyte-like cells (HLCs) from AM-MSCs (on differentiation day 12; HPCs) was investigated in vivo. For this, we established a two-chemical (CTX + TAA) model of acute liver failure in mice with improved stability of induction compared with the TAA-induced model. The AM-HPCs were successfully transplanted into the liver and spontaneously repopulate in the acute failure liver

Back to article page