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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Mesenchymal stem cell therapy ameliorates metabolic dysfunction and restores fertility in a PCOS mouse model through interleukin-10

Fig. 3

BM-hMSC injection into the ovary reverses metabolic phenotypes in the LTZ-induced PCOS mouse model. a Effect of LTZ on body weight in LTZ-treated mice (PCOS) and matched controls. Mean body weight after week 5 was significantly higher in the PCOS group (n=24) compared with matched controls (n=9). b Percent rate of increase in body weight in control mice (n=9) and PCOS mice (n=24). Graphs are presented as the mean ± SD. c Glucose tolerance test was performed on starved mice and mice after intraperitoneal (i.p.) glucose injection, monitored at the indicated time points for blood glucose level represented in mg/dL. d Blood glucose level at time 0 min and 120 min after glucose i.p. injection. BM-hMSC enhances energy expenditure in the PCOS mouse model. e Oxygen (O2), f carbon dioxide (CO2), g respiratory exchange ratio (RER), and h heat production presented in histograms comparing energy expenditure profiles of the PCOS group and BM-hMSC-treated PCOS group. ij BM-hMSC induces browning of white fat in the PCOS mouse model. i UCP-1 immunohistochemistry staining of white gonadal fat. Scale bar is 50μm. j Relative gene expression of UCP-1 (Ucp1), PGC-1α (Pgc1a), Cidea, and Prdm16 in white fat from PCOS mice and BM-hMSC-treated PCOS mice. *p<0.05, **p<0.005. ci All graphs are presented as the mean ± SD (n=3). Statistical significances were determined by 2-way ANOVA using GraphPad Prism 9

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