Fig. 10

Different suppression of NOS 2 expression and activity in activated THP-1 by between pMSCs-CM and UC-MSCs-CM. THP-1 monocytes in cultures were activated by the addition of PMA for 24 h, followed by the additional 24 h with LPS. The activated THP-1 cells were incubated with either pMSCs-CM or MU-MSCs-CM. a Nitric oxide (NO) levels from Griess assay. The NO level in each sample per an experiment was measured in triplicate and was represented by the average of the measurements. Data are presented as the mean ± SD of each group (n = 8 samples). The difference between groups was analyzed using two-tailed t-test. b NOS 2 protein levels in Western blot analysis. Data are representative of three independent experiments