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Fig. 9 | Stem Cell Research & Therapy

Fig. 9

From: Comparison of mesenchymal stromal cells from peritoneal dialysis effluent with those from umbilical cords: characteristics and therapeutic effects on chronic peritoneal dialysis in uremic rats

Fig. 9

Different protection of HPMCs from PDS-induced cell death by between pMSCs-CM and UC-MSCs-CM. A monolayer of HPMCs was exposed to 4.25% dextrose Dianeal PDS for 20 min, followed by 24-h incubation with either pMSCs-CM or UC-MSCs-CM. Cell viability/survival or apoptosis was measured using FACS analysis with staining with both 7-AAD and Annexin-V. a A typical FACS graph showing cell viability and cell death in each group. Q1: 7-AAD positive—necrotic cells; Q2 and Q3: Annexin-V positive—apoptotic cells; and Q4: both negative—viable cells. b Viability or survived cells in Q4. c Apoptosis (total of Q2 and Q3). The cell viability or apoptosis in each sample per an experiment was determined in duplicate and was represented by the average of the measurements. Data are presented as the mean ± SD of each group (n = 6 samples). The difference between groups was analyzed using two-tailed t-test

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