Fig. 1

Characterization of the ADSCs and the Glyoxalase-1 overexpression in ADSCs. P3-ADSCs in vitro were spindle-shaped and fibroblast-like under the microscope (A). Identification of the ADSC surface markers using flow cytometry showed that the cells were positive for CD29 and CD90, and negative for CD45 and CD34 (B). The G-ADSCs emitted green fluorescence under a fluorescence microscope (C). Western blot (D, E) and q-PCR (F) analyses proved the successful overexpression of the GLO-1 in the G-ADSCs. All experiments above were repeated three times (n = 3). A two-tailed Student’s t-test was performed to compare data between groups. *P < 0.05; **P < 0.01; scale bar = 100 μm. P3, passage 3; G-ADSCs, GLO-1-overexpressing adipose-derived stem cells