Fig. 2

Hypoxic preconditioning augments the neuroprotection of OM-MSCs in ICH animal model. A Schematic representation of the experimental design for in vivo experiments. B The rotarod test and C the modified neurological severity score (mNSS) test were performed before ICH and at 7, 14, and 28 days after ICH. Data are expressed as the mean ± SEM (n = 7/group at 7 days; n = 5/group at 14 and 28 days) (^***P < 0.001, ^∗∗P < 0.01 vs. sham-operated; ^#P < 0.05, ^##P < 0.01 vs. ICH + saline; ^&P< 0.01 vs. ICH + Normoxia MSCs). D Quantification of neuronal specific nuclear protein (NeuN) and terminal transferase-mediated dUTP nick end labeling (TUNEL) double-stained cells. Data are expressed as the mean ± SEM (n = 4) (^*P <0.05, ^**P <0.01, ^***P <0.001, ns. no significance). E Representative images of TUNEL staining in ipsilateral cortex after ICH. The TUNEL and NeuN double-positive cells were indicated with white arrows