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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Hypoxic preconditioning rejuvenates mesenchymal stem cells and enhances neuroprotection following intracerebral hemorrhage via the miR-326-mediated autophagy

Fig. 7

miR-326 attenuates cellular senescence of hypoxia OM-MSCs by promoting autophagy in vitro model. A Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of miR-326. B Western blotting and quantitative analysis of the expression levels of LC3II/I, Bcelin-1, and P62 in Hemin-exposed normoxia and hypoxia OM-MSCs. C Western blotting and quantitative analysis of the expression levels of LC3II/I, Bcelin-1, and P62 in normoxia OM-MSCs transfected with miRNA mimics control or miR-326 mimics. D Western blotting and quantitative analysis of the expression levels of LC3II/I, Bcelin-1, and P62 in hypoxia OM-MSCs transfected with miRNA inhibitor control or miR-326 inhibitor. E Western blotting analysis of LC3II/I, Bcelin-1, P62, p21, P53, and p16INK4A protein expression in normoxia MSCs transfected with miRNA mimics control, or miR-326 mimics, or miR-326 mimics +3MA. F Western blotting analysis of LC3II/I, Bcelin-1, P62, p21, P53, and p16INK4A protein expression in hypoxia MSCs transfected with miRNA inhibitor control, or miR-326 inhibitor, or miR-326 inhibitor + rapamycin. Data are expressed as the mean ± SEM (n = 3) (*P <0.05, **P <0.01, ***P <0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512