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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Divergence in chondrogenic potential between in vitro and in vivo of adipose- and synovial-stem cells from mouse and human

Fig. 2

In vivo chondrogenic differentiation of mASCs and mSSCs. A–C Mouse osteochondral model of non-transplanted, mASCs-transplanted, and mSSCs-transplanted group at day 21. Micro CT and histological images with the qualification for their positive stained area in the region of approximately 0.5 mm width and 0.4 mm depth are shown. Scale bars = 1 mm (μCT), 500 μm (Saf-O), and 100 μm (IHC images). Significance was assessed using one-way ANOVA with post hoc Tukey HSD test (a, p < 0.05 compared with defect; b, p < 0.05 compared with mASCs; c, p < 0.05 compared with mSSCs). D IHC for GFP counterstained with Alcian blue. Arrow heads indicate host derived cells. Representative data are shown (N = 5 for the GFP-mSSC transplantation group and N = 4 for the WT-mSSC transplantation). Scale bars = 50 μm. E Representative images for Saf-O and IHC for COL10 of the specimens of mSSC-transplanted group at 6 weeks. Break line indicates the area for neo-cartilaginous tissue. Scale bars = 50 μm. Significance of COL10-positive area between 3 and 6 weeks was assessed using Student’s unpaired t test (**, p < 0.01). F Micro CT images and their analysis for bone volume of total volume (approximately 0.5 mm width and 0.4 mm depth) and bone maturity calculated according to bone mineral component in bone volume. Scale bars = 1 mm. Significance was assessed using one-way ANOVA with post hoc Tukey honestly significant (HSD) difference test (*, p < 0.05; **, p < 0.01)

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