Fig. 3

TGFβ superfamily receptors and ligands in mASCs and mSSCs during chondrogenic culture. A, D Gene expression levels for TGFβ superfamily receptors and ligands in day 0 pellet of mSSCs and mASCs. Data are collected from over three independent lots and presented as a box plot with dot plot. Significance was assessed using Student’s unpaired t test (**, p < 0.01). B Western blot analysis of Smads in mSSCs and mASCs in chondrogenic culture. Representative images from two or three lots are shown. Signal intensity of pSmad1/5 and pSmad2 relative to GAPDH are shown as line graph which shape of points represent individual cell lots. C Saf-O images with the box plot for their positive stained area of histology from mSSCs and mASCs cultured with BMP2, TGFβ1, SB-431542 (SB), and/or LDN-193189 (LDN) for 14 days. Scale bars = 500 μm. Significance was assessed using one-way ANOVA with Dunnet test (*, p < 0.05; **, p < 0.01)