Fig. 2
Effects of hNTSC transplantation on Aβ protein load in 5 × FAD transgenic mouse brains. a hNTSCs or hBMSCs (1 × 105) were bilaterally injected into the hippocampus of 16-week-old 5 × FAD transgenic mice. AD neuropathological features were analyzed at 6 or 7 weeks after cell transplantation. b Confocal microscopy images of Tg-sham, Tg-hNTSC, and Tg-hBMSC brains after staining of OCT-embedded sections with an antibody against 6E10 to detect Aβ deposition (green) at 7 weeks after stem cell transplantation. Nuclei were labeled with DAPI (blue). Scale bars: 100 μm, 50 μm. c Sagittal and coronal PET images after [18F] flutemetamol injection in Tg-sham, Tg-hNTSC, or Tg-hBMSC mice at 6 weeks after stem cell transplantation. Increased signals for [18F] flutemetamol retention were detected in Tg-sham or Tg-hBMSC mice compared with Tg-hNTSC mice. d The 6E10-positive areas were quantified in the hippocampus and cortex (n = 5 per group). Values are the mean (SD). For the nonparametric multiple comparison tests, one-way ANOVA was used to determine whether group differences were statistically significant. *P < 0.05, **P < 0.01. e, f Aβ40 and Aβ42 levels in brain tissue lysates of Tg-sham, Tg-hNTSC, and Tg-hBMSC mice were analyzed by a specific Aβ ELISA at 7 weeks after stem cell transplantation (n = 5–6 per group). Values are the mean (SD). For the nonparametric multiple comparison tests, one-way ANOVA was used to determine whether group differences were statistically significant. *P < 0.05, **P < 0.01, ***P < 0.001. All images and data are representative of two or three independent experiments