Fig. 6

Effects of hNTSC transplantation on neuronal survival and the engraftment of hNTSCs in 5 × FAD mouse brains. a, b Confocal microscopy images of the brains of Tg-sham, Tg-hNTSC, and Tg-hBMSC mice after staining of brain tissue sections with an antibody against NeuN to detect neurons (green) at 7 weeks after stem cell transplantation. Nuclei were labeled with DAPI (blue). Scale bars: 1000 μm, 100 μm. c NeuN-positive cells were counted in the cortex (n = 5 per group). Values are the mean (SD). For the nonparametric multiple comparison tests, one-way ANOVA was used to determine whether group differences were statistically significant. **P < 0.01, ***P < 0.001. d Confocal microscopy images of the brains of Tg-hNTSC and Tg-hBMSC mice after staining of brain tissue sections with an antibody against HuNu to detect engrafted human cells (red) at 7 weeks after stem cell transplantation. Nuclei were labeled with DAPI (blue). Scale bars: 50 μm, 20 μm. e HuNu-positive cells were counted around the stereotaxic injection site in the brains (n = 4 per group). Values are the mean (SD). Statistical differences between two different samples were determined with Student’s t test. **P < 0.01. f, g Confocal microscopy images of the brains of Tg-hNTSC and Tg-hBMSC mice after double staining of brain tissue sections with antibodies against HuNu (red) and NeuN (green) at 7 weeks after stem cell transplantation. Nuclei were labeled with DAPI (blue). Scale bars: 20 μm, 10 μm. h HuNu-NeuN double-positive cells were counted in the cortex (n = 4 per group). Values are the mean (SD). Statistical differences between two different samples were determined with Student’s t test. *P < 0.05