Fig. 2

LPS-EEMos express an anti-inflammatory, immunosuppressive, and regenerative gene expression profile. Monocytes from 3 isolates were either untreated (control monocytes), treated for 24 h with unprimed MSC exosomes to produce EEMos, or with LPS-primed MSC exosomes to produce LPS-EEMos. After treatment, the cells were collected and RNA isolated and analyzed by qRT-PCR for gene expression. The fold change of gene expression normalized to a GAPDH housekeeping gene was compared to untreated control monocytes for A IL-6; B IL-8, IDO, and FGF2; and C IL-15, IL-10, IL-12, VEGFA, EGF, and IL-7. Results are from two replicates of three independent studies. Groups were compared by Kruskal-Wallis with a Dunn post-test. *p ≤ 0.05, ***p ≤ 0.0005, and **** p ≤ 0.0001 between groups are designated as compared to control monocytes