Fig. 7

Co-culture of LPS-EEMos with irradiated human CD34⁺ HSCs enhances viability and proliferation. Cryopreserved CD34⁺ HSCs isolated from G-CSF mobilized peripheral blood were thawed and irradiated with 4 Gy and labeled with VPD450 proliferation dye. The irradiated, VPD450-labeled CD34+ HSCs were then cultured alone or co-cultured in triplicate with LPS-EEMos, EEMos, or monocyte controls at a 1:1 ratio in a 96-well plate for 3 days. After 3 days, cells were stained for A Annexin V, CD34, and Ghost Dye™ Red 780 viability dye. Viable cells were identified as Annexin V⁻ Ghost Dye™ Red 780⁻. B Proliferation was determined by distribution of VPD450 in the CD34⁺ HSC population. Results compared by Kruskal-Wallis with Dunn’s post-test. Results are representative from 2 separate experiments, each using a different monocyte isolate. *p ≤ 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001