Table 1 Primer sequences for RT-PCR
Species | Gene | Primer sequence | Temperature |
|---|---|---|---|
Mouse | β-actin | For:TGGAATCCTGTGGCATCCATGAAAC Rev:TAAAACGCAGCTCAGTAACAGTCCG | 60 °C |
NLRP3 | For:GAGCTGGACCTCAGTGACAATGC Rev:ACCAATGCGAGATCCTGACAACAC | 60 °C | |
ASC | For:ACAATGACTGTGCTTAGAGACA Rev:CACAGCTCCAGACTCTTCTTTA | 58 °C | |
Caspase-1 | For:AGAGGATTTCTTAACGGATGCA Rev:TCACAAGACCAGGCATATTCTT | 58 °C | |
IL-1β | For:TCGCAGCAGCACATCAACAAGAG Rev:AGGTCCACGGGAAAGACACAGG | 60 °C | |
IL-18 | For:AGACCTGGAATCAGACAACTTT Rev:TCAGTCATATCCTCGAACACAG | 60 °C | |
IL-6 | For:AAGTCCGGAGAGGAGACTTC Rev:TGGATGGTCTTGGTCCTTAG | 58 °C | |
IL-10 | For:TTCTTTCAAACAAAGGACCAGC Rev:GCAACCCAAGTAACCCTTAAAG | 60 °C | |
TNF-α | For:AACTCCAGGCGGTGCCTATG Rev:TCCAGCTGCTCCTCCACTTG | 63 °C | |
Human | β-actin | For:CTCAGGAGGAGCAATGATCT Rev:GACCTGTACGCCAACACAGT | 60 °C |
NLRP3 | For:GCACTTGCTGGACCATCCTC Rev:GTCCAGTGCACACGATCCAG | 60 °C | |
ASC | For:CTCAAGAAGTTCAAGCTGAAGC Rev:TAGGTCTCCAGGTAGAAGCTG | 58 °C | |
Caspase-1 | For:GAAGAAACACTCTGAGCAAGTC Rev:GATGATGATCACCTTCGGTTTG | 58 °C | |
IL-1β | For:GCCAGTGAAATGATGGCTTATT Rev:AGGAGCACTTCATCTGTTTAGG | 60 °C | |
IL-18 | For:GCTGAAGATGATGAAAACCTGG Rev:CAAATAGAGGCCGATTTCCTTG | 60 °C |