Fig. 5
From: Low level laser therapy promotes bone regeneration by coupling angiogenesis and osteogenesis
LLLT enhancing osteogenic potential of the co-cultured hBMSCs and HUVECs during early stage of osteogenic induction. A HUVECs, hBMSCs, the co-cultured hBMSCs, and HUVECs were stained with ALP at 3 days and 7 days after osteogenic induction to assess their osteogenic ability in the early stage. B, C Quantification and analysis of the ALP staining by mean optical density (MOD) at 3 days and 7 days. D, E The expression level of the osteogenesis-related genes (ALP, RUNX-2) was measured by RT-qPCR at 3 days (D) and 7 days (E) after osteogenic induction (*p < 0.05, **p < 0.01, ***p < 0.001)