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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: A NOTCH1/LSD1/BMP2 co-regulatory network mediated by miR-137 negatively regulates osteogenesis of human adipose-derived stem cells

Fig. 4

HES1 knockdown promotes osteogenesis of hASCs in vitro. a, b ALP staining (a) and quantification (b) of transfected hASCs after a 7-day culture in PM or OM (scale bar = 100 μm). c, d ARS staining (c) and quantification (d) of transfected hASCs after a 14-day culture in PM or OM. e Relative expression analyses of HES1, RUNX2, ALP, and OCN by qRT-PCR in transfected hASCs on 3 days, 7 days, and 14 days. Data are shown as mean ± SD of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001 versus respective NC group

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