Fig. 5

NOTCH1 knockdown induces LSD1 while inhibits BMP2-SMADs pathway. a Relative expression analyses of LSD1, BMP2, and SMAD4 by qRT-PCR in transfected hASCs on 3 days, 7 days, and 14 days. b, c Western blotting (b) and band intensity analyses (c) of NOTCH1, LSD1, BMP2, SMAD4, and p-SMAD1/5 in transfected hASCs on 7 days. Data are shown as mean ± SD of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001 versus respective NC group