Fig. 1
Verification of tRF-5026a expression in tissues, plasma samples, and cells. a Electrophoretogram of tRF-5026a qRT-PCR products from two representative gastric cancer tissues. b T-A clone sequencing results of tRF-5026a qRT-PCR products with the original tRF-5026a sequence shown, the sequence of the two adaptors, and the crossing joint primer sequences. c Expression levels of tRF-5026a in gastric cancer tissues (n = 86). A larger ΔCq value indicates lower expression. d tRF-5026a (18 nt) and its mature tRNA (76 nt) were detected by Northern blotting. Three representative paired gastric cancer tissues (T) and adjacent noncancerous tissues (N) are shown. The 5S rRNA was used as a loading control. e The expression level of tRF-5026a was significantly downregulated in 79.07% (68/86) of gastric cancer tissues compared with normal tissues. f tRF-5026a levels in plasma samples from gastric cancer patients and healthy controls. A larger ΔCq value indicates a lower expression. n = 37. g tRF-5026a expression levels in cell lines. Low expression levels of tRF-5026a in gastric cancer cell lines were found compared with the normal gastric mucosal epithelial cell line GES-1. *P < 0.05, **P < 0.01, ***P < 0.001, ns, no significance