Fig. 3

In vitro MSC multilineage differentiation. Control group (A–E) and polytrauma group (F–J). Oil-Red staining (10×) after 21 days of culture (A/F). Alkaline phosphatase staining (10×) after MSC culture with baseline expansion medium (B/G) and osteodiff medium (C/H) for 10 days. Alizarin-Red staining (10×) with baseline expansion medium (D/I) and osteodiff medium (E/J) for 21 days. Scale bar: 100 μm