Fig. 2

miR-20a-5p facilitated osteogenic differentiation of hDPSCs in vitro. A Transfection efficiency in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups. B The relative expression of miR-20a-5p in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups at day 7. C The relative expression of miR-20a-5p in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups at day 14. D ALP staining in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups. E ALP activity in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups. F Quantification of ARS in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups. G The relative mRNA expression levels of RUNX2, OPN, and BSP in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups. H Protein levels of RUNX2, OPN, and BSP in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups. I Quantification of protein levels of RUNX2, OPN, and BSP in miR-20a-5p, anti-miR-20a-5p, and miR-NC groups. *p <0.05 and **p <0.01 compared with the miR-NC group