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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: miR-20a-5p contributes to osteogenic differentiation of human dental pulp stem cells by regulating BAMBI and activating the phosphorylation of Smad5 and p38

Fig. 5

miR-20a-5p regulated osteogenesis via targeting BAMBI. A ALP and ARS staining in si-NC and si-BAMBI groups. B ALP activity in si-NC and si-BAMBI groups. C Quantification of ARS in si-NC and si-BAMBI groups. D The relative mRNA expression levels of RUNX2 and BSP in si-NC and si-BAMBI groups. E Protein levels of RUNX2 and BSP in si-NC and si-BAMBI groups. F Quantification of protein levels of RUNX2 and BSP in si-NC and si-BAMBI groups. G ALP and ARS staining in miR-NC, anti-miR-20a-5p, si-BAMBI, and anti-miR-20a-5p plus si-BAMBI groups. H ALP activity in miR-NC, anti-miR-20a-5p, si-BAMBI, and anti-miR-20a-5p plus si-BAMBI groups. I Quantification of ARS in miR-NC, anti-miR-20a-5p, si-BAMBI, and anti-miR-20a-5p plus si-BAMBI groups. J The mRNA expressions of RUNX2 and OPN in miR-NC, anti-miR-20a-5p, si-BAMBI, and anti-miR-20a-5p plus si-BAMBI groups. K The protein levels of RUNX2 and OPN in miR-NC, anti-miR-20a-5p, si-BAMBI, and anti-miR-20a-5p plus si-BAMBI groups. L Quantification of protein levels of RUNX2 and OPN in miR-NC, anti-miR-20a-5p, si-BAMBI, and anti-miR-20a-5p plus si-BAMBI groups. *p <0.05 and **p <0.01 compared with the si-NC/miR-NC groups

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