Fig. 1

BK alleviated H₂O₂-induced cell apoptosis and ROS production in hCPCs. A Relative viability of hCPCs in the presence of different concentrations of H₂O₂ (100, 200, 300, 400, and 500 μM) detected with MTT. B Relative viability of the H₂O₂-induced hCPCs upon treatment with BK (1, 3, 10 nM) detected with MTT. C–D Representative image and quantitative analysis of B2 receptor in H₂O₂-induced hCPCs detected by western blot assay. E Representative image and quantitative analysis of TUNEL staining in hCPCs induced by H₂O₂ and treatment with 1, 3, 10 nM BK (Green staining cells indicated TUNEL-positive cells). F Representative image and quantitative analysis of in hCPCs induced by H₂O₂ and treatment with BK (1, 3, 10 nM) detected by Annexin V assay with flow cytometry. G Representative of image and quantitative analysis of flow cytometry detected the ROS production in hCPCs induced by H₂O₂ and treatment with BK (1, 3, 10 nM). H Representative of image and quantitative analysis of mitoSOX in hCPCs induced by H₂O₂ and treatment with BK (1, 3, 10 nM). (Data are shown as mean ± SEM, n = 5, *P < 0.05, **P < 0.01 VS. Control; #P < 0.05, ##P < 0.01 VS. H₂O₂.)