Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Mesenchymal stem cell-derived extracellular vesicles as probable triggers of radiation-induced heart disease

Fig. 3

Effects of hp-MSC-derived EVs on the proliferation and DNA damage of HUVEC and H9c2 cells after 72 h of culture. A Representative images of immunofluorescence analysis show Ki67-positive HUVEC or H9c2 cells. Scale bar: 50 μm. Quantitative data (n=3) indicate the percentage of Ki67-positive HUVEC cells (B) or H9c2 cells (C). D Representative images of immunofluorescence analysis show the DNA damage in HUVEC (53BP1 foci) or H9c2 (γ-H2AX foci) cells. Scale bar: 20 μm. Quantitative data (n=3) on the percentage of 53BP1-positive HUVEC cells (E) and γ-H2AX-positive H9c2 cells (F). Non-irradiated-EVs: EVs isolated from conditioned medium of non-irradiated hp-MSCs, Irradiated-EVs: EVs isolated from conditioned medium of irradiated hp-MSCs. Values are the mean ± SD. ns P > 0.5, *P < 0.5, **P < 0.1, ***P < 0.01, ****P < 0.001

Back to article page