Fig. 1
From: Mkx mediates tenogenic differentiation but incompletely inhibits the proliferation of hypoxic MSCs
The mRNA and protein expressions of Mkx in AMSCs and BMSCs at 7 days after induction. a qRT-PCR analysis of gene expression of Mkx under normoxia, Tgf-β1, hypoxia, and hypoxia+Tgf-β1 condition in AMSCs and BMSCs. Representative western blots (b) and quantification of protein expression (c) of Mkx under normoxia, Tgf-β1, hypoxia, and hypoxia+Tgf-β1 condition in AMSCs and BMSCs. Asterisks in the column in BMSCs indicated significantly different from the same condition in AMSCs. Representative immunofluorescence staining (d) of Mkx (red) and DAPI-labeled nuclei (blue), and quantification data (e) under normoxia, Tgf-β1, hypoxia, and hypoxia+Tgf-β1 condition in AMSCs and BMSCs. Asterisks in the column in BMSCs indicated significantly different from the same treatment condition in AMSCs. Scale bar = 50 μm; magnification, × 400. Data were shown as mean ± SD. ★p < 0.05; ★★p < 0.01