Fig. 3From: Mkx mediates tenogenic differentiation but incompletely inhibits the proliferation of hypoxic MSCsGross observation, western blot, and histological analysis of repaired patellar tendons at 4 weeks after surgery. a Gross observation, frozen sections observed by fluorescence microscopy, and Masson’s staining of the hypoxia and scramble group and the hypoxia and shRNA group. Scale bar = 200 μm; magnification, × 400. b Representative western blots of Col-1a1 and Col-3a1 of the hypoxia and scramble group and the hypoxia and shRNA group. c H&E staining and IHC staining for Col-1a1 and Tnmd of the hypoxia and scramble group and the hypoxia and shRNA group. H&E staining: scale bar = 200 μm; magnification, × 200; IHC staining: scale bar = 200 μm; magnification, ×400. d Histological scores of H&E staining and quantification of Col-1a1 and Tnmd after IHC staining in the hypoxia and scramble group and the hypoxia and shRNA group. Data were shown as mean ± SD. ★★p < 0.01Back to article page