Fig. 2
m6A level significantly decreased during osteogenic differentiation of hASCs. a Representative image of m6A level quantification assay. b Quantitative analyses of m6A level. c Expression of m6A regulators during the osteogenic differentiation of hASCs by RNA-seq (uhASCs vs dhASCs-7 day). d qRT-PCR analyses of the expression of METTL3, METTL14, FTO, and ALKBH5. e Western blot analysis of METTL3, METTL14, FTO, and ALKBH5 (uhASCs vs dhASCs-7 day). *p < .05, **p < .01, ***p < .001. hASCs, human adipose-derived stem cells; qRT-PCR, quantitative real-time polymerase chain reaction; uhASCs, undifferentiated hASCs; dhASCs, osteogenically differentiated hASCs