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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Transcriptome-wide m6A methylome during osteogenic differentiation of human adipose-derived stem cells

Fig. 5Fig. 5

Osteogenic pathways and genes were significantly differentially methylated and regulated. a Venn plot for DMEGs. b Four-quadrant graph for hyper-up, hypo-down, hyper-down, and hypo-up DMEGs. c Hierarchical clustering analysis of the top 50 DMEGs (by the standard fold change ≥ 2 and p < 0.05 for both DEGs and DMGs, ranking by p-value of DEGs). d GO enrichment analysis for DMEGs. e KEGG pathway analysis for DMEGs. f Hierarchical clustering analysis of DMEGs in the PI3K-Akt signaling pathway. g Hierarchical clustering analysis of DMEGs in the MAPK signaling pathway. h Hierarchical clustering analysis of DMEGs in the pathway of parathyroid hormone synthesis, secretion, and action. i Hierarchical clustering analysis of DMEGs in the P53 signaling pathway. j GSEA showed decreased enrichment of the Hedgehog signaling pathway. k GSEA showed decreased enrichment of the TGF-beta signaling pathway. DEGs, differentially expressed genes; DMGs, differentially methylated genes; DMEGs, differentially methylated and expressed genes; hASCs, human adipose-derived stem cells; uhASCs, undifferentiated hASCs; dhASCs, osteogenically differentiated hASCs; hyper-up, hypermethylated and upregulated genes; hypo-down, hypomethylated and downregulated genes; hyper-down, hypermethylated and downregulated genes; hypo-up, hypomethylated and upregulated genes; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; GSEA, gene set enrichment analysis

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