Fig. 4

Effect of ADSC-CM on DFSP cell migration by scratch wound healing. The average initial wound width was measured and defined as 100%. A Inverted microscopic images of DFSP cell wound repair. DFSP cells were treated with ADSC-CM (experimental), DMEM/F12+10% FBS (positive controls), or DMEM/F12 SF (negative controls). Wound healing within the scrape line was recorded at 0, 6, 12, and 24 h. Yellow dashed lines indicate the margin of the scratch. Scale bars=75 μm. Semi-quantification of migration rate at B 6 h, C 12 h, and D 24 h post-wounding. The wound areas were quantified in five random low-power fields per well using an inverted microscope. ^*p<0.05 and ^**p<0.01 indicate significant differences between the ADSC-CM and negative control groups in three independent experiments. ADSCs, adipose tissue-derived stem cells; CM, conditioned medium; DFSP, dermatofibrosarcoma protuberans; DMEM, Dulbecco’s modified Eagle’s medium; SF, serum-free