Fig. 5

Effect of ADSCs on DFSP cell migration by a Transwell assay. A The migratory DFSP cells were visualized by staining cells with crystal violet. Scale bars=50 μm. B Illustrations of Transwell co-culture systems in different conditions: DFSP cells were seeded onto the upper chambers with DMEM/F12+10% FBS (positive control), DMEM/F12 SF (negative control), or ADSCs (experimental) added to the lower chambers; then, cell migration was determined at 12 and 24 h. The numbers of migrated cells at 12 h (C) and 24 h (D). Migrated cells were counted in five random low-power fields per chamber using an inverted microscope. ^*p<0.05 and ^****p<0.0001 indicate significant differences between co-culture with the ADSCs (experimental) and negative control groups in three independent experiments. ADSCs, adipose tissue-derived stem cells; DFSP, dermatofibrosarcoma protuberans; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; SF, serum-free