Fig. 6

Effect of ADSC-CM on DFSP cell invasion by a Transwell assay with pre-coated Matrigel. A The invasive DFSP cells were visualized by staining with crystal violet. Scale bars=50 μm. B Illustration of cell invasion assay using Transwell with pre-coated Matrigel in different conditions. DFSP cells were seeded onto the upper chambers with DMEM/F12+10%FBS (positive control), DMEM/F12 SF (negative control), or ADSC-CM (experimental), and DMEM/F12+5%FBS was added to the lower chambers. Cell invasion was then determined at 36 h. C The number of invasive cells at 36 h. Invasive cells were counted in five random low-power fields per chamber using an inverted microscope. ^*p<0.05 indicates significant differences between the experimental and negative control groups determined in three independent experiments. ADSCs, adipose tissue-derived stem cells; CM, conditioned medium; DFSP, dermatofibrosarcoma protuberans; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; SF, serum-free