Fig. 5

Verification of MuSC proliferation and differentiation and regenerative muscle maturation by COL6 supplementation. a Sectional images of TA muscle 1 week after medium injection (Col6a1KO) or cell transplantation. Yellow arrows indicate Pax7+ nuclei. The inserts are higher magnifications. b Number of Pax7+ cells 1 week after the transplantation. n = 20 (medium; Col6a1KO), n = 6 (pMSCs), n = 10 (iMSCs), and n = 6 (KO-iMSCs). c Quantification of Pax7 + MyoD–, Pax7 + MyoD+, and Pax7–MyoD+ cell populations 1 week after the transplantation. Data are shown as the mean ± SD. n = 20 (medium; Col6a1KO), n = 6 (pMSCs), n = 10 (iMSCs), and n = 6 (KO-iMSCs). *P < 0.05 vs. KO, **P < 0.01 vs. KO, ^§P < 0.05 vs. iMSCs. d Sectional images of TA muscle 1 week after medium injection (COL6a1KO) or cell transplantation. Yellow arrows indicate MyoD+/Ki67+ nuclei. White arrow heads indicate MyoD+/Ki67- nuclei. Boxed areas indicated in the upper panels are shown with higher magnification in the lower panels. e Number of MyoD+ cells 1 week after the transplantation. n = 20 (medium; Col6a1KO), n = 6 (pMSCs), n = 10 (iMSCs), and n = 6 (KO-iMSCs). f % of Ki67+ cells per MyoD+ cells in the TA muscles of Col6a1KO mice 1 week after medium injection (Col6a1KO) or cell transplantation. n = 20 (medium; Col6a1KO), n = 6 (pMSCs), n = 10 (iMSCs), and n = 6 (KO-iMSCs). g Sectional images of TA muscles 1 week after iMSC transplantation. Yellow arrows indicate Pax7+ cells or MyoD+ cells. Data in b,e,f are shown as the mean ± SD. *P < 0.05. **P < 0.01. n.s., not significant