Fig. 6

Effect of COL6 protein on myogenesis in co-culture experiments. a Immunofluorescence images of Col6a1KO mouse-derived MuSCs 3 days after co-culture with pMSCs, iMSCs, or KO-iMSCs. Yellow arrowheads indicate MyoD+/Pax7- cells. b Number of DAPI+/human Lamin A/C- mouse MuSCs 3 days after co-culture. Data of three independent experiments are shown as the mean ± SD. **P < 0.01. c Percentage of Pax7 + MyoD–, Pax7 + MyoD+ and Pax7–MyoD+ cell populations 3 days after co-culture. Data of three independent experiments are shown as the mean ± SD. d Immunofluorescence images of Col6a1KO mouse-derived MuSCs 10 days after co-culture with pMSCs, iMSCs, or KO-iMSCs. Nuclei were stained with DAPI. e The area of MHC+ myotubes derived from mouse MuSCs 10 days after co-culture. Data of three independent experiments are shown as the mean ± SD. **P < 0.01. f Number of nuclei in MHC+ myotubes derived from mouse MuSCs 10 days after co-culture. Data of three independent experiments are shown as the mean ± SD. **P < 0.01. g The expression of marker genes. The mRNA expression of each gene was analyzed by RT-qPCR in primary MuSCs derived from Col6a1KO mice 6 days after co-culture with iMSCs or KO-iMSCs. Expressions are shown relative to values in MuSCs co-cultured with iMSCs for 6 days. MuSC marker: Pax7; myogenic markers: Myf5, Myod1, and Myogenin; myoblast fusion-related genes: Myomaker and Myomerger; muscle fiber-maturation markers: Myh8 and Myh1. Data of three independent experiments are shown as the mean ± SD. **P < 0.01