Skip to main content
Fig. 10 | Stem Cell Research & Therapy

Fig. 10

From: Adipose-derived stem cells alleviate radiation-induced dermatitis by suppressing apoptosis and downregulating cathepsin F expression

Fig. 10

ADSC culture supernatant downregulates the expression of CTSF, BAX, Bid, and caspase 9 and upregulates that of Bcl-2 and Bcl-XL in a radiation-induced apoptosis cell model in vitro. Representative blots of CTSF, BAX, Bid, Bcl-2, Bcl-XL, and caspase 9 levels (A, C). GAPDH was used as a loading control. Quantitative analysis of protein levels (B, D). I–III in A: I, NC-siRNA; II, CTSF-siRNA001; III, CTSF-siRNA002. I–VI in C: I, 20Gy-HaCaT+NC-siRNA; II, 20Gy-HaCaT+ ADSC-culture supernatant; III, 20Gy-HaCaT+CTSF-siRNA001; IV, 20Gy-HaCaT+ CTSF-siRNA002; V, 20Gy-HaCaT+ADSC-culture supernatant+CTSF-siRNA001; VI, 20Gy-HaCaT+ADSC-culture supernatant+CTSF-siRNA002. The results are expressed as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. NC-siRNA group

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512