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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Bone marrow mesenchymal stem cells promote remyelination in spinal cord by driving oligodendrocyte progenitor cell differentiation via TNFα/RelB-Hes1 pathway: a rat model study of 2,5-hexanedione-induced neurotoxicity

Fig. 3

Hes1 expression and its relationship with the BMSCs-induced remyelination in HD-exposed rats. a qRT-PCR results showing Hes1 mRNA level in the spinal cords of HD/BMSCs-treated rats. b Western blot results showing Hes1 protein level in the spinal cords of HD/BMSC-treated rats. c Representative images showing MBP (red) and Hes1 (green) staining in the spinal cords of HD/BMSCs-treated rats. Scale bar, 50 μm. d Quantification of MBP+ and Hes1+ cells. e Western blot results showing Hes1 protein expression in cultured OPCs treated with HD/HD + BMSC-CM. f, g qRT-PCR and western blot results showing Hes1 mRNA and protein levels in Hes1-overexpressing OPCs but not vector-transfected OPCs. h Representative phase images of cultured OPCs treated with HD/HD + BMSC-CM/HD + BMSC-CM + vector/HD + BMSC-CM + Hes1 overexpression. Yellow arrows represent OPCs, green arrows represent mature OLs, and cyan arrows represent immature OLs. Scale bar, 50 μm. i Quantification of OPCs, immature OLs, mature OLs and death cell. j Representative images of MBP staining in the above described OPCs. Scale bar, 50 μm. k Quantification of MBP+ cells. l Western blot results showing MBP level. Quantified data are shown as mean ± SD. a: Compared with control group, p < 0.05; b: compared with HD group, p < 0.05; c: compared with HD + BMSCs group, p < 0.05

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