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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Bone marrow mesenchymal stem cells promote remyelination in spinal cord by driving oligodendrocyte progenitor cell differentiation via TNFα/RelB-Hes1 pathway: a rat model study of 2,5-hexanedione-induced neurotoxicity

Fig. 5

TNFα expression and its relationship with the BMSCs-induced remyelination in HD-exposed rats and OPCs via Hes1 regulation. a, b qRT-PCR and western blot results showing TNFα mRNA and protein levels in the spinal cords of HD/BMSCs-treated rats. c Western blot results showing TNFα level in HD/BMSC-CM-treated OPCs. d Representative phase images of HD/BMSC-CM-treated OPCs ± TNFα treatment. Yellow arrows represent OPCs, cyan arrows represent immature OLs, and green arrows represent mature OLs. Scale bar, 50 μm. e Quantification of OPCs, immature OLs, mature OLs and death cell. f Representative images showing MBP staining in HD/BMSC-CM-treated OPCs ± TNFα. Scale bar, 50 μm. g Quantification of MBP+ cells. h Western blot results showing MBP and Hes1 expression in HD/BMSC-CM-treated OPCs ± TNFα. Quantified data are shown as mean ± SD. a: Compared with control group, p < 0.05; b: compared with HD group, p < 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512