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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Bone marrow mesenchymal stem cells promote remyelination in spinal cord by driving oligodendrocyte progenitor cell differentiation via TNFα/RelB-Hes1 pathway: a rat model study of 2,5-hexanedione-induced neurotoxicity

Fig. 6

RelB expression and its relationship with the BMSCs-induced remyelination in HD-exposed rats and OPCs via Hes1 regulation. a, b qRT-PCR and western blot results showing RelB mRNA and protein levels in the spinal cords of HD/BMSCs-treated rats. c Western blot showing RelB expression in OPCs treated with HD/HD + BMSC-CM/HD + BMSC-CM + TNFα. d, e qRT-PCR and Western blot showing overexpression of RelB in OPCs. f Representative phase images of vector-transfected OPCs or RelB-overexpressing OPCs treated with HD/HD + BMSC-CM. Yellow arrows represent OPCs, cyan arrows represent immature OLs, and green arrows represent mature OLs. Scale bar, 50 μm. g Quantification of OPCs, immature OLs, mature OLs and death cell. h Representative images showing MBP staining in vector-transfected OPCs or RelB-overexpressing OPCs receiving HD/HD + BMSC-CM treatment. Scale bar, 50 μm. i Quantification of MBP+ cells. j, k Western blot results showing MBP and Hes1 expression in vector-transfected OPCs or RelB-overexpressing OPCs in response to aforementioned treatment. Quantified data are shown as mean ± SD. a: Compared with control group, p < 0.05; b: compared with HD group, p < 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512