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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: RETRACTED ARTICLE: GDF11 enhances therapeutic functions of mesenchymal stem cells for angiogenesis

Fig. 3

GDF11 enhanced angiogenic paracrine activity of MSCs. A The angiogenesis-related proteins from MSCVector and MSCGDF11 were analyzed by a cytokine antibody array. B Quantitative analysis of protein levels in A. C Quantification of VEGF in the conditioned medium of MSCGDF11 and MSCVector by ELISA. D Western blot analysis of VEGFA and HGF proteins in MSCs after MSCs was transduced with vectors carrying gene for GDF11 or control (vector). E Quantitative analysis of protein levels in E. F Quantification of exosomes form MSCsvector, MSCGDF11, MSCsvector + GW4869, and MSCGDF11 + GW4869 by NTA. N = 5. G The protein concentration per 108 particles in MSCGDF11 and MSCVector. N = 5. H Western blot analysis of exosomal proteins and proangiogenic cytokines in exosomes from MSCs and their conditioned media in which exosomes were eliminated. VEGF and HGF were higher in both exosomes and media from MSCGDF11 than from MSCVector

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