Fig. 4From: Translational models of 3-D organoids and cancer stem cells in gastric cancer researchEffect of ROCK inhibitor in gastric organoid culture. The rock inhibitor supplied in culture medium maintained the growth and morphologies of the organoids well and helped to avoid the culture-damages caused by the culture-environments. (a) The cultivation in the absence of the ROCK inhibitor (left lane) caused the growth of the organoids slowly. (b) Addition of ROCK inhibitor (10 µM; right panel) resulted in the growth of the organoids 1.5–2.0-fold faster compared with the control organoids (left panel). Human adenocarcinoma of stomach organoid lines, HCM-BROD-0208-C16 cancer model primary adenocarcinoma of stomach was purchased from American Type Culture Collection (PDM-146™; ATCC, VA, USA) and cultured as its recommended protocol [5] (bright view, scale bar = 100 μm). (c) The stomach organoids were generated by seeding in 96-well plates and grown 2 days ROCK inhibitor (n = 6): Th organoids were incubated with CellTiter-Glo®3D reagent (G9681, Promega, Madison, WL, USA) prior to culture medium addition, and results were acquired to assay (** P < 0.01).Back to article page